LOCUS       40924_49077        3149 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD013, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3149)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3149)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    370..375
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    complement(373..393)
                     /note="C2G; GC-adaptor for seamless assembly, flanking 
                     antibiotic resistance markers, functional devices or custom
                     sequences; synthetic"
     misc_feature    394..399
                     /label=AgeI
                     /note="AgeI"
     CDS             504..1100
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     regulatory      1112..1138
                     /note="trpA T; trpA terminator from Lorist6 INSD accession 
                     X98450, one nt shorter than reference; Lorist6"
                     /regulatory_class="terminator"
     misc_feature    1148..1153
                     /label=NheI
                     /note="NheI"
     misc_feature    complement(1154..1174)
                     /note="GC; GC-adaptor for seamless assembly, flanking
                     antibiotic resistance markers or Escherichia coli origins 
                     to be assembled with a cyanobacterial replicon; synthetic"
     misc_feature    1172..1177
                     /label=EcoRV
                     /note="EcoRV"
     rep_origin      complement(1402..1990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2164..3021)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3022..3126)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccgcatg atatccggcg gcggcggcgg gataccggtt tcgaatgtct caaaatctct
      421 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
      481 taaacagtaa tacaaggggt catatgactg aatataaacc cactgttcgt ttagctactc
      541 gtgatgatgt tccacgtgct gttcgtactt tagctgctgc ttttgctgat tatccagcta
      601 ctcgtcatac tgttgatcca gatcgtcata ttgaacgcgt gactgaattg caagaactgt
      661 ttttaactcg cgtgggtctg gatattggta aagtttgggt tgctgatgat ggtgctgctg
      721 ttgctgtttg gactactcca gaaagtgttg aagctggtgc tgtttttgct gaaattggcc
      781 cacgtatggc tgaattaagt ggtagtcgtt tagctgctca acaacaaatg gaaggtttat
      841 tagctccaca tcgtccaaaa gaaccagctt ggtttttagc tactgttggt gttagtccag
      901 atcaccaagg taaaggttta ggtagtgctg ttgttttacc aggtgtagaa gctgctgaac
      961 gtgctggtgt tccagctttt ttagaaacaa gtgctccacg caatctgcca ttctatgaac
     1021 gtttaggttt taccgttacc gctgatgttg aagttcctga aggcccacgt acttggtgta
     1081 tgactcgtaa accaggtgct taaggccggc cagcccgcct aatgagcggg cttttttttg
     1141 gcgcgccgct agcatccgcg cgcgcgcgcg cgatatcctg ggcctcatgg gccttccgct
     1201 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaacat ggtcatagct
     1261 gtttccttgc gtattgggcg ctctccgctt cctcgctcac tgactcgctg cgctcggtcg
     1321 ttcgggtaaa gcctggggtg cctaatgagc aaaaggccag caaaaggcca ggaaccgtaa
     1381 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1441 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1501 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1561 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1621 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1681 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1741 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1801 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1861 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1921 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1981 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2041 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2101 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2161 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2221 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2281 cagtgctgca atgataccgc gagaaccacg ctcaccggct ccagatttat cagcaataaa
     2341 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2401 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2461 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2521 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2581 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2641 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2701 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2761 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     2821 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     2881 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     2941 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3001 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3061 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3121 tccgcgcaca tttccccgaa aagtgccac
//
